Immunoelectron microscopy. Compound, frozen in chilled acetone, cut into 6 sections, and incubated first with anti GFP antibody overnight at 4 and then with horseradish peroxidase conjugated goat rabbit IgG F(ab 2 (BioSource International, Camarillo, CA) for 2 h at room temperature. The sections were then fixed for an additional 5 min in 1% glutaraldehyde, reacted with DAB and H2O2, postfixed in 2% osmium tetroxide for 60 min, dehydrated in a graded ethanol series, and embedded in Epon 812 resin.

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