The brown module with the most highly significant biofunctions in the IC31 group included categories of biofunctions that are directly linked to adaptive immune responses, such as humoral immune response and cell mediated immune response. Furthermore, many of the other categories, such as cellular development and hematological system development, contained biofunctions associated with the initiation of adaptive immune responses, including proliferation of lymphocytes and quantity of lymphocytes, respectively. Pou class 2 associating factor 1 (Pou2af1), a molecule shown to play an important role in multiple stages of B cell development and GC formation in mice upon immunization with T cell dependent antigen20,21, was one of the transcripts involved in most biofunctions of adaptive immunity (Fig.

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