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Endogenous pro meltrin (115 kDa) and processed mature meltrin (86 kDa) were also immunoprecipitated (Figure 7a). Immunoblotting with the anti phosphotyrosine mAb PY20 showed that Tyr of the MelCyt was phosphorylated when v Src was transfected (Figure 7b). This phosphorylation took place on Tyr901 because MelCyt(Y901F) was not phosphorylated.

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